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KMID : 0366119740020020127
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Korean Journal of Applied Microbiology & Bioengineering
1974 Volume.2 No. 2 p.127 ~ p.131
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Inhibition of Over-oxidation of 11β-17α, 21-trihydroxy-pregna-1, 4-diene-3, 2-dione in Fermentative Process
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Bae Moo
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Abstract
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Inhibition of over-oxidation of prednisolone in the fermentation has been studied by using vegetative cells as enzyme source.
Firstly, A. simplex (ATCC 6946) was demonstrated to degrade prednisolone in the vegetative culture of the microorganism.
Over 72% of hydrocortisone was transformed into prednisoloneby 3 hours of the fermentation However, the prednisolone produced was considerably oxidized forming over-oxidation product in 8hours of fermentation period with the intact cells.
Secondly, in order to depress the over-oxidation and the breakdown of the steroid skeleton. of prednisolone, chelating agents such as αα´-dipyridyl, o-phenanthroline and 8-hydroxyquinoline were added to the fermentation broth. Consequently, the breakdown of prednisolone by the intact cells was able to be remarkably retarded and an intermediate regarded as an oxidized product derived from prednisolone was accumulated, by the addition of αα´-dipyridyl in the fermentation.
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